The calibration probe is an option that ships with the TSQ Series II Plus models. If you have a second generation TSQ Series II mass spectrometer, you can order the calibration probe from Thermo Fisher Scientific.

NOTE

The calibration probe is not compatible with the EASY-Max NG API source or the Ion Max NG API source.

If you have a calibration probe, Thermo Fisher Scientific recommends that you use it instead of the H-ESI probe when you calibrate the MS, as the calibration probe is specifically designed for this purpose. After you run the calibration routine, uninstall the calibration probe and reinstall the H-ESI probe.

The installation procedure for the calibration probe is the same as the installation procedure for the H-ESI probe.

Procedure

  1. Adjust the probe height between L and M, except for the TSQ Endura MS.
  2. NOTE

    The TSQ Endura MS uses the EASY-Max NG API source, which does not provide any height adjustment for the probe.

  3. Adjust the front-to-back position of the probe (typically near position 1 and 1.5 for a syringe flow rate from 2 to 5 μL/min).
  4. Center the left-to-right position of the probe.
  5. Probe position for the calibration process
    123
    No.DescriptionNo.Description

    1

    Depth position (z axis) between M and L

    3

    Left-to-right position (x axis) at the center position

    2

    Front-to-back position (y axis) at a position slightly less than 1