Set up syringe pump with the calibration solution - TSQ Quantis Plus MS - TSQ Fortis Plus MS - TSQ Series - TSQ Altis MS - TSQ Quantis MS - TSQ Altis Plus MS - TSQ Fortis MS - Set up syringe pump with the calibration solution - TSQ Quantis Plus MS - TSQ Fortis Plus MS - TSQ Series - TSQ Altis MS - TSQ Altis MS - TSQ Quantis MS - TSQ Quantis MS - TSQ Altis Plus MS - TSQ Fortis MS

Procedure

1. Fill the syringe with a sufficient amount of fresh calibration solution and verify that the calibration solution flows through the tubing and H-ESI needle without resistance.
2. The System Calibration process takes 30 to 50 minutes with the syringe pump set to a flow rate from 3 to 5 μL/min.

3. IMPORTANT

   Nitrile gloves are made with diphenylguanidine, which is soluble in EMRS. If diphenylguanidine contaminates the EMRS, the spectrum includes a peak at m/z 212.

   Thermo Fisher Scientific recommends wearing other chemically resistant gloves when working with the EMRS.

4. IMPORTANT

   To prevent the EMRS from degrading, do the following:

   • Do not return any EMRS back to the original vial.
   • Do not store the EMRS in a glass syringe. Discard unused the EMRS and rinse the syringe with acetonitrile.
   • If you observe degradation products in the EMRS mass spectrum, rinse the sample line (including the fittings, syringe, tubing, and union) with acetonitrile.

5. NOTE

   Observe these storage precautions for the EMRS and reserpine solutions:

   • Refrigerate the containers after opening.
   • or long-term storage, keep the containers refrigerated at 2–8 °C (36–46 °F)
2. If the calibration solution does not flow through the tubing and needle without resistance, do the following:
1. Make new clean cuts at both ends of the PEEK tubing.
2. Inspect the tip of the needle insert (preferably under a microscope) for dirt or damage.
3. If necessary, replace the H-ESI needle insert. Use the tool to ensure correct needle protrusion.
4. For information about setting up the syringe and connecting it to the sample transfer line, see Syringe and syringe pump setup.
3. When you connect the sample transfer line to the ion source housing’s grounding union make sure that the union is made of stainless steel, as solvent leaks between the sample transfer line and the union can create an electric shock hazard if the union is not properly grounded.

4. CAUTION

   To prevent electric shock, verify that the grounding union is made of stainless steel. A grounding union made of a non-conductive material, such as PEEK, creates an electric shock hazard.