The following figure shows the manual loop injection plumbing using the optional divert/inject valve with an APCI configuration.

Figure Manual loop injection configuration with optional divert/inject valve
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No.DescriptionNo.Description

1

To waste

4

To APCI spray insert sample inlet

2

Needle injection port

5

From divert/inject valve (port 6)

3

Loop

6

From LC pump

NOTE

The metal grounding union is not required in the plumbing path of an APCI configuration.

 

Required Materials

  • Divert/inject valve (not provided)

Procedure

  1. Connect a sample loop to the divert/inject valve (ports 1 and 4).
  2. Connect a loop filler (needle port) to the divert/inject valve (port 2).
  3. Connect Teflon tubing to the divert/inject valve (port 3) using a Rheodyne fitting. Then, connect the remaining end into an appropriate waste container.
  4. Connect red PEEK tubing from the LC pump’s outlet to the divert/inject valve (port 5) using a one-piece fingertight fitting.
  5. Connect red PEEK tubing using a one-piece fingertight fitting to the divert/ inject valve (port 6). Then, connect the remaining end to the APCI spray insert’s sample inlet using a two-piece fingertight fitting.
  6. Load a clean syringe with the sample solution and carefully insert it into the needle port.

  7. NOTE

    To perform a manual loop injection, slowly press the syringe plunger.