The connection between the LC system or the syringe and the spray insert can be as follows:
- For standard flow applications, use a Viper capillary or red PEEK tubing.
- For low flow applications, install the ESI spray insert. Connect the appropriate solvent line tubing from the LC system or the syringe pump to the metal grounding union. Then, use natural PEEK tubing between the metal grounding union and the spray insert’s sample inlet.
- For nanoflow applications, use a nanoViper capillary.
CAUTION
Risk of electric shock
- Do not bypass the metal grounding union when connecting the LC system to the API source. Bypassing the grounding union creates an electric shock hazard to the user and other connected devices.
- If you observe a liquid leak from the tubing or fitting, turn off the voltages to the API source housing by setting the instrument to Standby mode before you troubleshoot the leak. The components and liquid might be electrically conductive and could cause an electric shock when touched.
The following figure shows the plumbing for the H-ESI configuration.
| No. | Description | No. | Description |
|---|---|---|---|
1 | From autosampler or LC pump | 3 | To H-ESI spray insert |
2 | Metal grounding union |
Procedure
- Insert a metal grounding union into the grounding union holder.
CAUTION
Risk of electric shock
To prevent electric shock, verify that the grounding union is made of stainless steel metal. A grounding union made of a nonconductive material, such as PEEK, creates an electric shock hazard.
- (Optional) Install an LC column on the HPLC.
- Connect red PEEK tubing to the outlet of the autosampler or LC pump with an appropriate fitting and ferrule. Then, connect the remaining end of the red PEEK tubing to the metal grounding union using a two-piece fingertight fitting.
- Connect red PEEK tubing using a two-piece fingertight fitting to remaining end of the metal grounding union. Then, connect the remaining end to the H-ESI spray insert’s sample inlet using a one-piece fingertight fitting.