Open the Method Editor Application - Stellar MS - Stellar Mass Spectrometer Operating Manual - Open the Method Editor Application - Method Editor Application - Software - Stellar MS
Stellar Mass Spectrometer Operating Manual
Product Tree
Mass Spectrometry > LC-MS Mass Spectrometers > Stellar MS
Document Type
Operating Manual
Software version
1.0 QF1
Stellar Mass Spectrometer
Preface
Access Documentation
Use the Data System Computer
Open the Help System
Special Notices, Symbols, and Cautions
Contact Us
Introduction
Stellar MS
Overview of an LC/MS Analysis
Ionization Modes
ESI Modes (H-ESI and ESI)
H-ESI Mode for Analysis
ESI Mode for Auto-Ready Calibration
APCI Mode
APPI Mode
NSI Mode
Hardware Components
External Features
Dimensions and Weight
Exterior Panels
LED Indicators
Communications Panel
Power Entry Module
Cooling Fans
Ion Optics
Main Components
Vacuum System and Inlet Gases
Vacuum Pumps
Vacuum Manifold
Vacuum Gauges
Inlet Gas Supply Hardware
Gas Specifications
Helium Gas Regulator
Nitrogen Gas Valves
Sheath Gas, Auxiliary gas, and Sweep gas
Vent Valve
API Source
Compatible Ion Sources and Interfaces
API Source Interface
Ion Sweep Cone
Spray Cone
Ion Transfer Tube
Heater Block
Vent Prevent Ball
RF Lens and Exit Lens
MP00 Ion Optics
MP0 Ion Optics
Mass Analyzers
Quadrupole Mass Analyzer
Mass Analysis
Applied RF and DC Fields
Quadrupole Offset Voltage
Linear Ion Trap Mass Analyzer
Overview
Axial Trapping Voltages
AC Voltages Applied to the X-rods
Helium Damping Gas
Ion Concentrating Routing Multipole
Collision Cell and HCD Efficiency
Quadrupole Offset Voltage
MP3 Ion Optics
Ion Detection System
Software
Tune Application
Tune Application Window
Buttons and Icons
Open the Plot Chromatogram Dialog Box
Parameters in the Plot Chromatogram Dialog Box
Chromatogram View
Spectrum View
Parameters in the Spectrum View
Data Acquisition Controls
Options Menu
Tune Preferences
Parameters in the Tune Preferences
Generate Reports
Tune Panes
Ion Source Pane
Define Scan Pane
Calibration Pane
Status Area
Calibration Area
Instructions Area
Status Pane
History Pane
Favorites Pane
Diagnostics Pane
Alerts Console
Specify the Alert Settings
Alerts Console Control Options
Method Editor Application
Open the Method Editor Application
Global Parameters Page
Scan Parameters Page
Method Layouts and Templates
Quan View
Add a Scan Type to the Experiment Workflow
Hybrid View
Add a Scan Type to the Experiment Workflow
Templates View
Save a Custom Template
To Set the Recommended API Source Settings
Check API Source Settings for the LC Flow Rate
H-ESI Source Settings
APCI Source Settings
NSI Source Settings
Scan Parameters
Scan Types
Full Scan
SIM Scan
MSn Scan
SRM Scan
Ion Polarity Modes
Data Types
Data Acquisition
Use the Xcalibur Data System to Acquire Data
Select the Start Instrument
Use the Tune Application to Acquire Data
MongoDB Database
Set the Historical Data Retention
iAPI License
Installation
To Connect the Forepump to the Vacuum System
Connect the Forepump to the MS
Connect the Forepump to the Lab Exhaust System
Connect the Drainage System on the MS
Connect the Nitrogen and Helium Gas Lines
To Set up the Syringe Pump and Divert/inject Valve
Syringe Pump
Set up the Syringe
Divert/inject Valve
Configurations
Divert/inject Valve Controls
Set up the Syringe Pump and Divert/inject Valve Holding Bracket
Connect the Cables and Power Cords
Control an External Device using Contact Closure
To Set up the API Source
Remove the API Source
Install or Remove the Ion Sweep Cone
Install the API Source
Prepare the Spray Insert for the API Source
H-ESI spray insert
APCI Spray Insert
Remove or Install the API Spray Insert
Spray Insert Position and Adjustments
Adjust the Spray Insert Position
Auto-Ready Enclosure
Check the Auto-Ready Components
To Connect the Inlet Plumbing
Guidelines for PEEK Tubing and Fittings
Plumbing Schematic Overview
Plumbing Setups for an HPLC
Set up the Plumbing for a H-ESI Configuration
Set up the Plumbing for an APCI Configuration
Set up the Plumbing for Direct Infusion
Set up the Plumbing for High-flow Infusion (without an autosampler)
Plumbing Setups for Manual Loop Injections (flow-injection analysis)
Set up the Loop Injection Plumbing for a H-ESI Configuration
Set up the Loop Injection Plumbing for an APCI Configuration
Operation
Mobile Phase Buffer Guidelines
LC Additive Guidelines
System Startup, Shutdown, and Reset
On/Off Status of MS Components
To Turn on the MS
Turn on the MS from the Tune Application
Turn on the MS from the Data System
Place the MS in Standby Mode
Shut Down the MS
Shut Down the MS in an Emergency
Restart the MS
Reset the MS
Daily Operation
Before Operating the MS
Check the Vacuum Pressure Levels
Find and Fix Air Leaks
Check the Gas Supplies
After Operating the MS
Flush the Inlet Components
Empty the Solvent Waste Container
Place the System in Standby Mode
Calibration
Calibration Overview
Pierce FlexMix Calibration Solution Peak Values
Auto-Ready Calibration
Prepare the Auto-Ready Hardware for Calibration
Calibrate using Auto-Ready
Calibration in ESI Mode using a Syringe Pump
Set up the MS for Calibration
Establish a Stable Ionization Spray
Calibrate in ESI Mode
To Flush the Inlet Components After Calibration
Recommended Supplies
Flush the Inlet Components
Maintenance
Guidelines
Tools and Supplies
Maintenance Schedule
Maintain the API Source Housing
To Maintain the External Components of API Source Interface
To Maintain the Ion Sweep Cone
Remove the Ion Sweep Cone
Clean the Ion Sweep Cone
Reinstall the Ion Sweep Cone
To Maintain the Ion Transfer Tube
Remove the Ion Transfer Tube
Clean the Ion Transfer Tube
Reinstall the Ion Transfer Tube
Maintain the Spray Cone and O-ring
To Maintain the Internal Components of the API Source Interface
Remove the API Source Interface
Remove the RF Lens, Exit Lens, MP00 RF Rods, and L0 Lens
Clean the RF Lens, Exit Lens, MP00 RF Rods, and L0 Lens
Reinstall the RF Lens and Exit Lens
Reinstall the MP00 RF Rods and L0 Lens
Reinstall the API Source Interface
Clean the Air Filter
Replace the Auto-Ready Calibration Solution Vial
Replaceable Parts
Preinstallation Kit
Chemical Kit
UHPLC/MS Installation Kit
Installation Kit
Calibration Kit
Accessory Kit
Setup Kit
High Volume Dry Pump Kit
API Source Interface
Miscellaneous
Legal Notices
Copyright and Trademarks
Regulatory Compliance
Regulatory Compliance Results
FCC Compliance Statement
Notice on the Use and Handling of Thermo Scientific Instruments
WEEE Directive 2012/19/EU
Glossary
From the Windows taskbar, select Start → Thermo Scientific Xcalibur → Xcalibur . Select the Roadmap View icon, if applicable. Select Instrument Setup .