API Source Interface - Stellar MS - Stellar Mass Spectrometer Operating Manual - API Source Interface - Main Components - Hardware Components - Stellar MS

Stellar Mass Spectrometer Operating Manual
Product Tree
Mass Spectrometry > LC-MS Mass Spectrometers > Stellar MS
Document Type
Operating Manual
Software version
1.0 QF1
  • Stellar Mass Spectrometer
  • Preface
  • Access Documentation
  • Use the Data System Computer
  • Open the Help System
  • Special Notices, Symbols, and Cautions
  • Contact Us
  • Introduction
  • Stellar MS
  • Overview of an LC/MS Analysis
  • Ionization Modes
  • ESI Modes (H-ESI and ESI)
  • H-ESI Mode for Analysis
  • ESI Mode for Auto-Ready Calibration
  • APCI Mode
  • APPI Mode
  • NSI Mode
  • Hardware Components
  • External Features
  • Dimensions and Weight
  • Exterior Panels
  • LED Indicators
  • Communications Panel
  • Power Entry Module
  • Cooling Fans
  • Ion Optics
  • Main Components
  • Vacuum System and Inlet Gases
  • Vacuum Pumps
  • Vacuum Manifold
  • Vacuum Gauges
  • Inlet Gas Supply Hardware
  • Gas Specifications
  • Helium Gas Regulator
  • Nitrogen Gas Valves
  • Sheath Gas, Auxiliary gas, and Sweep gas
  • Vent Valve
  • API Source
  • Compatible Ion Sources and Interfaces
  • API Source Interface
  • Ion Sweep Cone
  • Spray Cone
  • Ion Transfer Tube
  • Heater Block
  • Vent Prevent Ball
  • RF Lens and Exit Lens
  • MP00 Ion Optics
  • MP0 Ion Optics
  • Mass Analyzers
  • Quadrupole Mass Analyzer
  • Mass Analysis
  • Applied RF and DC Fields
  • Quadrupole Offset Voltage
  • Linear Ion Trap Mass Analyzer
  • Overview
  • Axial Trapping Voltages
  • AC Voltages Applied to the X-rods
  • Helium Damping Gas
  • Ion Concentrating Routing Multipole
  • Collision Cell and HCD Efficiency
  • Quadrupole Offset Voltage
  • MP3 Ion Optics
  • Ion Detection System
  • Software
  • Tune Application
  • Tune Application Window
  • Buttons and Icons
  • Open the Plot Chromatogram Dialog Box
  • Parameters in the Plot Chromatogram Dialog Box
  • Chromatogram View
  • Spectrum View
  • Parameters in the Spectrum View
  • Data Acquisition Controls
  • Options Menu
  • Tune Preferences
  • Parameters in the Tune Preferences
  • Generate Reports
  • Tune Panes
  • Ion Source Pane
  • Define Scan Pane
  • Calibration Pane
  • Status Area
  • Calibration Area
  • Instructions Area
  • Status Pane
  • History Pane
  • Favorites Pane
  • Diagnostics Pane
  • Alerts Console
  • Specify the Alert Settings
  • Alerts Console Control Options
  • Method Editor Application
  • Open the Method Editor Application
  • Global Parameters Page
  • Scan Parameters Page
  • Method Layouts and Templates
  • Quan View
  • Add a Scan Type to the Experiment Workflow
  • Hybrid View
  • Add a Scan Type to the Experiment Workflow
  • Templates View
  • Save a Custom Template
  • To Set the Recommended API Source Settings
  • Check API Source Settings for the LC Flow Rate
  • H-ESI Source Settings
  • APCI Source Settings
  • NSI Source Settings
  • Scan Parameters
  • Scan Types
  • Full Scan
  • SIM Scan
  • MSn Scan
  • SRM Scan
  • Ion Polarity Modes
  • Data Types
  • Data Acquisition
  • Use the Xcalibur Data System to Acquire Data
  • Select the Start Instrument
  • Use the Tune Application to Acquire Data
  • MongoDB Database
  • Set the Historical Data Retention
  • iAPI License
  • Installation
  • To Connect the Forepump to the Vacuum System
  • Connect the Forepump to the MS
  • Connect the Forepump to the Lab Exhaust System
  • Connect the Drainage System on the MS
  • Connect the Nitrogen and Helium Gas Lines
  • To Set up the Syringe Pump and Divert/inject Valve
  • Syringe Pump
  • Set up the Syringe
  • Divert/inject Valve
  • Configurations
  • Divert/inject Valve Controls
  • Set up the Syringe Pump and Divert/inject Valve Holding Bracket
  • Connect the Cables and Power Cords
  • Control an External Device using Contact Closure
  • To Set up the API Source
  • Remove the API Source
  • Install or Remove the Ion Sweep Cone
  • Install the API Source
  • Prepare the Spray Insert for the API Source
  • H-ESI spray insert
  • APCI Spray Insert
  • Remove or Install the API Spray Insert
  • Spray Insert Position and Adjustments
  • Adjust the Spray Insert Position
  • Auto-Ready Enclosure
  • Check the Auto-Ready Components
  • To Connect the Inlet Plumbing
  • Guidelines for PEEK Tubing and Fittings
  • Plumbing Schematic Overview
  • Plumbing Setups for an HPLC
  • Set up the Plumbing for a H-ESI Configuration
  • Set up the Plumbing for an APCI Configuration
  • Set up the Plumbing for Direct Infusion
  • Set up the Plumbing for High-flow Infusion (without an autosampler)
  • Plumbing Setups for Manual Loop Injections (flow-injection analysis)
  • Set up the Loop Injection Plumbing for a H-ESI Configuration
  • Set up the Loop Injection Plumbing for an APCI Configuration
  • Operation
  • Mobile Phase Buffer Guidelines
  • LC Additive Guidelines
  • System Startup, Shutdown, and Reset
  • On/Off Status of MS Components
  • To Turn on the MS
  • Turn on the MS from the Tune Application
  • Turn on the MS from the Data System
  • Place the MS in Standby Mode
  • Shut Down the MS
  • Shut Down the MS in an Emergency
  • Restart the MS
  • Reset the MS
  • Daily Operation
  • Before Operating the MS
  • Check the Vacuum Pressure Levels
  • Find and Fix Air Leaks
  • Check the Gas Supplies
  • After Operating the MS
  • Flush the Inlet Components
  • Empty the Solvent Waste Container
  • Place the System in Standby Mode
  • Calibration
  • Calibration Overview
  • Pierce FlexMix Calibration Solution Peak Values
  • Auto-Ready Calibration
  • Prepare the Auto-Ready Hardware for Calibration
  • Calibrate using Auto-Ready
  • Calibration in ESI Mode using a Syringe Pump
  • Set up the MS for Calibration
  • Establish a Stable Ionization Spray
  • Calibrate in ESI Mode
  • To Flush the Inlet Components After Calibration
  • Recommended Supplies
  • Flush the Inlet Components
  • Maintenance
  • Guidelines
  • Tools and Supplies
  • Maintenance Schedule
  • Maintain the API Source Housing
  • To Maintain the External Components of API Source Interface
  • To Maintain the Ion Sweep Cone
  • Remove the Ion Sweep Cone
  • Clean the Ion Sweep Cone
  • Reinstall the Ion Sweep Cone
  • To Maintain the Ion Transfer Tube
  • Remove the Ion Transfer Tube
  • Clean the Ion Transfer Tube
  • Reinstall the Ion Transfer Tube
  • Maintain the Spray Cone and O-ring
  • To Maintain the Internal Components of the API Source Interface
  • Remove the API Source Interface
  • Remove the RF Lens, Exit Lens, MP00 RF Rods, and L0 Lens
  • Clean the RF Lens, Exit Lens, MP00 RF Rods, and L0 Lens
  • Reinstall the RF Lens and Exit Lens
  • Reinstall the MP00 RF Rods and L0 Lens
  • Reinstall the API Source Interface
  • Clean the Air Filter
  • Replace the Auto-Ready Calibration Solution Vial
  • Replaceable Parts
  • Preinstallation Kit
  • Chemical Kit
  • UHPLC/MS Installation Kit
  • Installation Kit
  • Calibration Kit
  • Accessory Kit
  • Setup Kit
  • High Volume Dry Pump Kit
  • API Source Interface
  • Miscellaneous
  • Legal Notices
  • Copyright and Trademarks
  • Regulatory Compliance
  • Regulatory Compliance Results
  • FCC Compliance Statement
  • Notice on the Use and Handling of Thermo Scientific Instruments
  • WEEE Directive 2012/19/EU
  • Glossary

The API source interface components are located in a vacuum chamber, except for the atmospheric pressure side of the spray cone and the nose cone of the ion transfer tube.

Figure API source interface
API source interface


The following topics describe the components of the API source interface:

  • Ion Sweep Cone
  • Spray Cone
  • Ion Transfer Tube
  • Heater Block
  • Vent Prevent Ball
  • RF Lens and Exit Lens
  • MP00 Ion Optics

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