The following figure shows the Calibration pane with Auto-Ready.
| No. | Description | No. | Description |
|---|---|---|---|
1 | Colored status indicator | 4 | Recommended calibration date and last successful calibration date |
2 | Calibration tab | 5 | FlexMix volume status |
3 | Polarity status toggle | 6 | Calibration options (Mode and Polarity) |
Prerequisites
- Check the FlexMix volume from the Calibration pane. A minimum volume of 5% (approximately 250 µL) is required to perform an instrument calibration. The system prevents starting a calibration routine when the minimum volume is below that value.
Procedure
- Open the Tune application. For instructions, see Tune Application Window.
- Select the Options
icon and then select Preferences. - In the Current Calibration Options area, select Auto-Ready as the calibration source.
- (Optional) In the System Self-Check Options area, select the checkbox to enable the system self-check option to schedule periodic instrument calibration checks.
- Select OK to exit the Tune Preferences dialog box.
- The system automatically sets FlexMix as the calibration solution.
- From the Tune application, select the Calibration pane.
- In the Calibration area, select one of the following options, as applicable:
- Mode:
- - Check, Calibrate if required (recommended)
- - Calibrate
- Polarity: Positive or Positive and Negative
- Select Start.
- The calibration operation begins with the selected options. After the system has completed a successful calibration, the Calibration pane resets to show the date of the next recommended calibration for each calibration set.
NOTE
Thermo Fisher Scientific recommends that you include a shutdown method at the end of every sequence that uses an LC workflow when scheduling Auto-Ready calibrations with the Self-Check feature. The Self-Check process automatically adjusts system settings, such as the source gases, temperature settings, and so on.
Include the following actions in the shutdown method:
- Set the vaporizer temperature to 0 °C
- Set the LC flow rate to under 10 µL/min
- Set source gases to minimal levels, which helps reduce source housing dryness