Run the calibration from the Tune application, starting with positive polarity and then continuing with negative polarity using the FlexMix calibration solution (P/N A39239).

Prerequisites

  1. Load a clean 500 µL syringe with at least 250uL of the FlexMix calibration solution.
  2. Set up the Plumbing for Direct Infusion
  3. Set up the MS for Calibration
  4. Establish a Stable Ionization Spray

Procedure

  1. Open the Tune application.
  2. Select the Options icon and then select Preferences.
  3. The Tune Preferences dialog box appears.
  4. In the Current Calibration Options area, select Other as the calibration source. Then select OK to close the dialog box.
  5. From the Tune application, select the Calibration pane.
  6. In the Calibration area, select one of the following options as applicable:
  7. Mode:
  8. - Check, Calibrate if required (recommended)
  9. - Calibrate
  10. Polarity: Positive or Negative
  11. Select Start.
  12. The calibration operation begins. After the system has completed a successful calibration, the status area in the Calibration pane resets to show the date of the next recommended calibration for each calibration set.
  13. Repeat this procedure in negative ion mode.

IMPORTANT

Before you start using your analyte, follow the procedure in To Flush the Inlet Components After Calibration.