Plumbing Setups for Manual Loop Injections (flow-injection analysis) - Stellar MS - Stellar Mass Spectrometer Operating Manual - Installation - To Connect the Inlet Plumbing - Plumbing Setups for Manual Loop Injections (flow-injection analysis) - Stellar MS

Use the loop injection technique when there is a limited amount of sample. To use this technique, attach a sample loop, an injection port fitting (needle port), and an LC pump to the divert/inject valve, and then connect the valve to the API source.

With the divert/inject valve in the 1–2 (Load) position, use a syringe to load sample through the needle port into the sample loop, and then switch the position of the inject valve to the 1–6 (Inject) position. Switching the valve to the Inject position allows the solvent flow from the LC pump to backflush the sample out of the loop and into the API source.

Follow these guidelines:

• Use a manual loop injection without chromatographic separation for qualitative or quantitative analysis when there is a limited amount of a pure sample.
• Use a manual loop injection with chromatographic separation for qualitative or quantitative analysis when there is a limited amount of a sample mixture. This requires an LC column between the injection valve and the API source.

Follow the appropriate procedure based on your system configuration:

• Set up the Loop Injection Plumbing for a H-ESI Configuration
• Set up the Loop Injection Plumbing for an APCI Configuration