Flush the Inlet Components - Stellar MS - Stellar Mass Spectrometer Operating Manual - Daily Operation - Flush the Inlet Components - Operation - After Operating the MS - Stellar MS

Prerequisites

1. Wear personal protective equipment (PPE) such as a lab coat, safety glasses, and chemical resistant gloves.

Procedure

1. Open the Tune application and turn off the syringe pump, if applicable.
2. From the Tune application, select System Standby to place the MS in Standby mode.
3. Remove the syringe from the syringe pump as follows:
1. Press and hold the pusher block release button, then move the pusher block to the left.
2. Unfasten the syringe clamp from the syringe.
3. Remove the syringe from the syringe-holder block channel.
4. Remove the syringe needle from the Teflon tube on the syringe adapter assembly.

5. NOTE

   Anytime you remove the syringe needle from the Teflon tubing, always loosen the fingertight fitting first to avoid strain on the needle tip during removal.

4. Rinse the syringe with a solution of 50:50 methanol-water, or an appropriate solvent for your assay.
5. Remove the sample transfer line, sample tube, and spray insert from the API source housing.
6. Flush the sample transfer line, sample tube, and spray insert as follows:
1. Load the clean syringe with a solution of 0.1% formic acid in 50:50 methanol-water (or another appropriate solvent, such as a solution of 0.1% formic acid in 50:50 acetonitrile-water).
2. Hold the syringe plunger in place and carefully insert the tip of the syringe needle into the open end of the Teflon tubing on the syringe adapter assembly.
3. Slowly depress the syringe plunger to flush the sample transfer line, sample tube, and spray insert into a solvent waste container
4. Reinstall the sample transfer line, sample tube, and spray insert.