Nonlinear response and loss of sensitivity may occur from carbonate contamination. Thermo Fisher Scientific recommends a freshly prepared 1M base treatment under these conditions for the Eluent Suppressor component. Wear appropriate safety gear during this operation.

Procedure

  1. Disconnect the lines to the Eluent Suppressor and perform the following treatment on the Eluent Suppressor component.
  2. Push 3 mL of 1 M NaOH using a syringe through ELUENT IN port and divert a line out of ELUENT OUT port to waste.
  3. Push 5 mL of 1 M NaOH using a syringe through REGEN IN and divert a line out of REGEN OUT port to waste.
  4. Allow the Eluent Suppressor unit to equilibrate for 20 minutes
  5. Repeat steps 2 and 3 with DI water and reinstall the unit on the system.
  6. Repeat treatment for a longer time if problem persists.