The following table contains known defects in the software, categorized by software section, with a brief abstract and information related to each defect’s severity and risk. The Item ID is the internal number assigned to each issue. Product management assesses risk, which can differ significantly from the reported severity.

Software section

Severity

Abstract

Risk

Item ID

Statistics Insights node

Low

The Stats Configuration contents should be shown under the Statistics Insights node for the Result Summaries page.

Low

817550

Predicted library export

Medium

Tribrid instrument, Eclipse, Spectrum Library shown FT as Mass Analyzer.

Low

823209

Protein FDR

Medium

Protein FDR validation cannot be complete when PEP scores are zero.

Med

828198

Predicted library

Low

When predicting the MS library using a peptide list exported from the Proteome Discoverer application, the Normalized Collision Energy parameter of the Spectrum Library Prediction dialog box is not automatically filled with the value retrieved from the CSV file.

Low

830121

Scripting node

Low

When exporting tables with the Scripting node, if there are two columns with the same display name and purpose, only one is exported.

Low

830428

Transition list editor, Minora Targeted Detector

Medium

Before starting the analysis, validate the transition list when the modification is out of range.

Low

832141

Spectrum view

Medium

Mass error does not accurately reflect when the mass tolerance is increased.

Med

832632

Spectrum view

Medium

Annotated peaks are incorrect.

Med

832635

SureQuant nodes

Low

Reading the boundaries file created from Proteome Discoverer by Skyline failed because the peptide contained unrecognized modifications, for example, xxx[Carbbamidoethyl]xxx.

Low

834323

TMT

Low

It is better to show the Scan Type (such as Full or Zoom) in the result tables of TMTpro.

Low

799432

Predicted library

Medium

Add the spectrum library filter information in the Comment column.

Low

814963

Isolation interference

Medium

Quan Spectrum isolation interference should account for when HR scan window is different (0.7mz) compared to the ID MS2 spectrum (1.2 mz).

Med

844042

ProSightPD

Medium

The proteins in the top-down results cannot be exported in the mzIdentML format or the mzML format.

Med

846212

Modifications

Medium

The Modifications (All Possible Sites) of some peptide groups does not stay the same when repeating a workflow multiple times.

Low

847212

Study

Low

When editing names in the Input Files column, duplicate names should not be allowed.

Low

854066

Help

Low

When pressing CTRL+F1 on the keyboard to display the Help information for results tables, the target page is not displayed. Rather, the Interpreting Search Results with the Results File is displayed.

Low

855517

Ardia

Medium

The MSF file downloaded from an Ardia server cannot be used as results for a consensus analysis.

Med

856927

Minora Targeted Detector

Medium

The Minora Targeted Detector node does not allow for partial reprocessing with CHIMERYS.

Low

857300

Transition list editor, Minora Targeted Detector

Low

When loading a transition list that includes peptide sequence annotation (for example, (light), (heavy)), there is no dialog that allows the correct peptide sequences.

Low

868506

Result Filter/View

Medium

Editing Tags does not immediately carry over to the filter view.

Low

746930

Ardia

Low

Cancelling an upload to Ardia leads to an upload failed message.

Low

791045

Targeted peptide quantifier

Low

There is no ability to view the processing log when invalid lines are found while loading the transition list with the peptide fragment filters in the Targeted Peptide Quantifier node of the consensus workflow.

Low

867622

Spectrum selector

Low

The Acquisition Type parameter in the Spectrum Selector node shows a very technical error message.

Low

815428

Library prediction

Medium

Library predictions from a peptide list contain more peptides (same sequence, same modifications, different charge state) than requested.

Low

848258

Sever settings

Low

Need more information for the Max. Number of Processing Workflows in Parallel Execution.

Low

851958

Mascot

Low

The job should be aborted after the Mascot analysis failed.

Low

842791

DIA results

Medium

In DIA experiments, the normalization of DIA precursors, sequences, and isoforms should take the normalization factors calculated for the peptide groups.

Low

861908

Library prediction

Medium

The invalid INFERYS peptides should be filtered out when you save a CSV peptide list from a .raw file generated from a transition list.

Low

862120

Transition list editor

Medium

When loading a transition list with fragment ion information and peptides where the precursor m/z are close (delta m/z is less than 0.01), the fragment ions listed will be the union of all peptides within that range.

Low

855715

CHIMERYS on Ardia

Medium

When the search space for the CHIMERYS on Ardia sever exceeds the limit, there is no hint information to reduce the search space, only an exit code.

Low

754229

CHIMERYS

Medium

The Proteome Discoverer application allows the combination of CHIMERYS results with different quan values, and in the DIA Precursors tables, the values for Area and Intensity are not reported in separate columns.

Med

713421

Ardia

Medium

Multiconsensus results generated in Proteome Discoverer 3.3 from remote MSF files from Proteome Discoverer 3.2 provide no quan ratios in the result. There is workaround to use the Proteome Discoverer 3.2 application to produce multiconsensus results or use remote .raw files to create new MSF files.

Med

827042

Reporter ion quantification

Medium

The Minimum Channel Occupancy parameter does not filter as expected.

Med

869823

PTM annotation

Low

The modification string for a protein might be different when multiple search engines are used.

Med

870723

Spectral annotation

Medium

The PSM Details dialog box does not show the predicted spectrum when the peptide has a met-loss modification.

Low

866816

Export

Medium

When exporting the library in a FASTA format, there are only peptide sequences in the exported file, but no mass list, which is unexpected.

Med

784270

Targeted peptide chromatograms

Low

The plot of Chromatogram Traces View cannot be toggled among a sequence at the Targeted Precursor Groups, its Targeted Precursors, and its Targeted Fragment Peak.

Low

845542

PSM table

Low

The PSMs orders are different in the PSMs table when repeating the analysis.

Low

869748