The following table lists defects that were resolved between Proteome Discoverer 3.2 and Proteome Discoverer 3.3. The table excludes Help issues and any cosmetic fixes. In some cases, the original abstract has been amended or expanded upon to better describe the reported issue. Both an engineering fix and follow-up testing (verified by our product evaluation department) have resolved each of these issues.

Item ID

Software section

Abstract

207794

Help

Documentation: A better description for INFERYS and spectral library prediction functionality is needed.

792818

Reporter ion quantification

TMT 32plex: Some values in the PSM are duplicated in the quan channel view plot.

842421

Reporter ion quantification

When using the precursor detector and reporter quantifier, the quan spectra are used for PSMs with high isolation interference.

93039

Normalization

Enable users to normalize only features found in all sample groups in quan analysis.

851668

Ardia

When users are not registered on any Ardia server: For studies containing many analysis files, the repeated check of remote files might slow down the performance.

842518

PSM filtering

Low-confident PSMs are still present when they belong to a high-confident peptide group.

824138

Spectral libraries

Predict from a FASTA file: The Activation Type parameter always shows HCD even when the CID option is selected during library creation.

838825

Spectrum selector

The workflow crashes when the Spectrum Selector node tries to store traces for the .raw files.

794155

Familiarization documentation

Familiarization Guide: In Chapter 2 for reporter ion quantification with phosphorylation, the Peptide Groups data source needs to be removed from the Volcano Plot steps.

798301

Fragment ions quantifier

The numbering index of the parameters is not correct in the Fragment Ions Quantifier node.

802042

Spectrum view

The Fragment Chart displays only charge 1 for DIA spectra.

802419

Protein Annotation node

When the database version changed, the workflow's annotation node and search engine node do not recognize the new version of the database, and the workflows need to be updated.

806818

MSPepSearch

MSPepSearch InforHint displays an incorrect warning about RAM memory limitations.

277263

Reporter ion quantification

An incorrect warning that reporter ion quantification cannot rescale abundance columns displays.

562597

Dependencies

The application does not use SQLite 118.

647791

Magellan server

Local databases (for example, Server DB) are insecure and not encrypted. Users can compromise settings and data integrity is not enforced.

746695

Percolator

Update to Percolator 3-07.

752141

Study

The word “Hints” does not appear in the the Execute State column in the analysis result table for jobs that completed with hints.

756957

CHIMERYS communication tester

Verify Discoverer can get an object from S3 and there are no network misconfigurations on the client side.

763521

FASTA file download

A FASTA file with and without subtaxonomies with the same TaxId cannot be downloaded.

772816

Protein table

The values of the #Protein Unique Peptides column in the Proteins table are 0.

777000

Ardia

If the default browser is not set to Microsoft Edge, then the sign in to Ardia server will not work.

778296

Processing

Processing workflow failed: Caller is unauthorized to delete specified resource[0].

782322

CHIMERYS

The CHIMERYS node failed because it cannot process spectra.

758266

Export

Astral .raw files exported to .mgf files contain no precursor mass in cases where the precursor detector is used.

856182

Reporter ion quantification

TMT quan values might be swapped when no sample grouping is used.

856230

Label free quan results

When the Precursor Ions Quantifier node is used, the chromatogram for the DIA Precursors table is missing.

764341

Plots

Originally,hidden tables (even after being made visible) cannot be used to plot histograms.

797992

CHIMERYS on Ardia

A gateway timeout occurred when submitting a job that resulted in a conflict.

829338

INFERYS library prediction, export, and MSPepSearch

Non-target peptides are added to the predicted library as targets when predicting from a protein FASTA file. The results failed MSPepSearch searches using a target/decoy due to insufficient target/decoys needed for FDR.

799390

CHIMERYS cloud

CHIMERYS skips the final merge step and downloads results for input files separately, reducing the length of time to finalize the result.

802042

Spectrum view

Only singly charged fragments are annotated for DIA spectra.