The following table lists the parameters in the Volcano Plots view.

Parameters in the Volcano Plots view

Parameter

Description

Data Source

Specifies the type of data to plot from:

  • Proteins: From protein data.
  • Peptide Groups: From peptide group data.

Comparison

Specifies the items to be compared.

P-value

Sets the p-value, which is the likelihood that abundances of a particular peptide across different samples are not different. For information on p-values, see Calculate p-values and adjusted p-values for quantification results.

Drag the slider to change the y-axis range of the shaded areas, or enter the appropriate number in the box to the right of the slider and press the ENTER key.

Log2 Fold Change

Sets the Log2 fold change, which is the ratio of the difference between the last value and the first value over the original value. (If the initial value is A and final value is Z, the fold change is (Z - A)/A or Z/A - 1.)The fold change is expressed in log2 format.

Drag the slider to change the x-axis range of the shaded areas, or enter the appropriate number in the box to the right of the slider and press the ENTER key.

Log10 P-value (y axis)

Displays p-values in log10.

Log2 Ratio (x axis)

Displays the abundance ratio in log2.

Legend

Displays the meaning of the colors of groups of proteins or peptide groups.

By default, the legend is hidden. Select Show Legend from the shortcut menu to display the legend.