Procedure

  1. In the Protein Identification Details view, select the Coverage tab.
  2. When the site probability scores are available (that is, when you included the ptmRS node in the processing workflow), the application color-codes the scores.
  3. From the Threshold list in the PTM Site Probabilities area, select the category of probability for a modification to occur on a sequence site.
  4. For example, when you choose 25 from the Threshold list, the Protein Identification Details view displays modifications that have a low (25–100%) probability of occurrence on a site.
  5. NOTE

    The application does not display modifications with less than a 25% chance of occurring on a site.

  6. Protein Identification Details view showing modifications with a low probability of occurring on a site
  7. To display only those modifications with a very high probability (99–100%) of a modification occurring on a site, select 99 from the Threshold list.
  8. Protein Identification Details view showing modifications with a high probability of occurring on a site