The following table describes the Predict a Spectrum Library parameters.
Parameters | Description |
|---|---|
General | |
FASTA File | Select the FASTA file that you want to use to create the predicted spectral library. |
Library Name | The name of the predicted spectrum library. |
Organism | The name of the organism described in the FASTA file. |
Activation Type | Specifies the activation type: HCD or CID |
Collision Energy | Specifies the collision energy value. Range: 10–50; default: 28 |
Comment | Optional field for user notes. |
Digest | |
Enzyme | Specifies the name of the FASTA database. |
Min. Peptide Length | Specifies the minimum peptide length. Range: 7–30; default: 7 |
Max. Peptide Length | Specifies the maximum peptide length. |
Min. Precursor Charge | Specifies the minimum precursor charge. Range: 2–6; default: 2 |
Max. Precursor Charge | Specifies the maximum precursor charge. Range: 2–6; default: 3 |
Maximum Missed Cleavage Sites | Specifies the minimum mass of the precursor ion. Range: 0–12; default: 2 |
Static Modification | Specifies the static modifications that the application applies to every instance of the specified residues or terminus. |
Dynamic Modification | Specifies a known or suspected modification. Dynamic modifications might or might not be present. |
Max. Equal Modifications per Peptide | Specifies the maximum number of times that the same dynamic modification can be used in a single peptide. Range: 0–20; default: 3 |