If all your searches using the Mascot search engine fail, follow these instructions to locate the problem.
Procedure
- Verify that the Mascot server is running and accessible from the computer that is running the Proteome Discoverer application.
- For details, see Connect to the Mascot server.
- To verify that the Mascot server is operating properly, submit a simple search from the Mascot web interface, and then do one of the following:
- If the search from the Mascot web interface is successful, continue to the next step.
- If the search fails, contact your system administrator. There might be a problem with the Mascot server itself.
- If your Mascot server is operating properly and you can access it from the Proteome Discoverer application, perform a simple search using the Mascot node, and then do one of the following as applicable:
- If simple searching fails, there might be a general problem in the interaction between the Proteome Discoverer application and the Mascot server. In this case, file a bug report.
- If you can perform simple Mascot searches, investigate your failing searches more closely by considering these questions:
- - Does the search finish successfully on the Mascot server according to the Mascot search log?
- - Do the process messages that are sent to the job queue during the search indicate the problem?
- If these suggestions still do not resolve the search problem, file a bug report.
- See Contact us.