With the Scan Event Filter node, you can divide data from a “mixed-mode” experiment so that each “mode” can be processed independently. For example, if you have a raw data file obtained from alternating ETD and CID scans, you might want to analyze the CID portion of the data with the Sequest HT search engine and the ETD portion with the Mascot search engine.
The following table describes the parameters for the Scan Event Filter node.
Parameter | Definition |
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Mass Analyzer | Specifies the type of mass analyzer used for the acquisition of the spectrum:
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MS Order | Specifies the level of the mass spectrum to be processed, for example, MS/MS or MS3. Range: MS1–MS10; default: Any |
Activation Type | Specifies the fragmentation method used to activate the scan:
For descriptions, see Multidimensional separation experiments. |
Min. Collision Energy | Filters out the spectra of ions fragmented with low collision energy from the spectra of ions fragmented with high collision energy. This parameter filters out values below the specified value. Range: 0–100; default: 0 |
Max. Collision Energy | Filters out the spectra of ions fragmented with high collision energy from the spectra of ions fragmented with low collision energy. This option filters out values above the specified value. Range: 1–1000; default: 1000 |
Scan Type | Specifies the scan type to be used for the search:
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Polarity Mode | Specifies the polarity of the spectrum.
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