With the Scan Event Filter node, you can divide data from a “mixed-mode” experiment so that each “mode” can be processed independently. For example, if you have a raw data file obtained from alternating ETD and CID scans, you might want to analyze the CID portion of the data with the Sequest HT search engine and the ETD portion with the Mascot search engine.

The following table describes the parameters for the Scan Event Filter node.

Scan Event Filter node parameters

Parameter

Definition

Mass Analyzer

Specifies the type of mass analyzer used for the acquisition of the spectrum:

  • Ion Trap
  • Fourier Transform
  • Time of Flight
  • Single Quad
  • Triple Quad
  • Sector Field
  • (Default) Any

MS Order

Specifies the level of the mass spectrum to be processed, for example, MS/MS or MS3.

Range: MS1–MS10; default: Any

Activation Type

Specifies the fragmentation method used to activate the scan:

  • CID (Collision-Induced Dissociation)
  • MPD (Multi-Photon Dissociation)
  • ECD (Electron Capture Dissociation)
  • PQD (Pulsed Collision-Induced Dissociation)
  • ETD (Electron Transfer Dissociation)
  • HCD (High-Energy Collision Dissociation)
  • EThcD (ETD with supplemental HCD)
  • UVPD (Ultraviolet Photodissociation)
  • (Default) Any Activation Type

For descriptions, see Multidimensional separation experiments.

Min. Collision Energy

Filters out the spectra of ions fragmented with low collision energy from the spectra of ions fragmented with high collision energy. This parameter filters out values below the specified value.

Range: 0–100; default: 0

Max. Collision Energy

Filters out the spectra of ions fragmented with high collision energy from the spectra of ions fragmented with low collision energy. This option filters out values above the specified value.

Range: 1–1000; default: 1000

Scan Type

Specifies the scan type to be used for the search:

  • Full: Scans the full mass range to acquire the data. The mass spectrometer detects a wide range of mass fragments. Used for typical data-dependent scanning.
  • Selected Ion Monitoring: Scans a very small mass range to acquire the data. The mass spectrometer detects only certain mass fragments.
  • Selected Reaction Monitoring: Scans a single mass in the first analyzer and a single user-defined fragment ion in the second analyzer to acquire the data. The mass spectrometer detects a specific product ion of a specific parent ion.
  • (Default) Any: Uses any of the three scan types just described.

Polarity Mode

Specifies the polarity of the spectrum.

  • Positive: Specifies a positive polarity.
  • Negative: Specifies a negative polarity.
  • (Default) Any: Specifies either polarity.