The following table lists the enzymes and reagents with cleavage properties.
Enzymes/Reagents | Cleaves after | Cleaves before | Except when |
|---|---|---|---|
Enzymes for digestion | |||
AspN |
| D |
|
Chymotrypsin | F, W, Y, or L |
|
|
Chymotrypsin (FWY) | F, W, or Y |
| P is after F, W, or Y |
Clostripain | R |
|
|
Elastase | A, L, I, or V |
| P is after A, L, I, or V |
Elastase/Tryp/Chymo | A, L, I, V, K, R, W, F, or Y |
| P is after Al, L, I, V, K, R, W, F, or Y |
GluC | E or D |
|
|
LysC | K |
|
|
No-Cleavage |
|
|
|
No-Enzyme | Every amino acid |
|
|
Proline_Endopept | P |
|
|
Staph_protease | E |
|
|
Trypsin | K or R |
| P is after K or R |
Trypsin (KRLNH) | K, R, L, N, or H |
|
|
Trypsin_K | K |
| P is after K |
Trypsin_R | R |
| P is after R |
Chemicals for degradation | |||
Cyanogen bromide | M |
|
|
Iodobenzoate | W |
|
|