Including Precursor Detector node - Proteome Discoverer Software - Including Precursor Detector node - Proteome Discoverer Software - Proteome Discoverer Software

You can create a workflow that handles chimeric, or mixed spectra, by including the Precursor Detector node for datasets not currently supported by CHIMERYS. The node supports identifying additional precursors within the isolation window of the precursor spectrum in the results.

Use in workflows that process data detected by Orbitrap MS/MS detected data. For search nodes, use the Sequest HT node or the MSPepSearch node

For an example results page that shows chimeric peaks, see The MS/MS Spectrum Info page.

Special Processing Workflow Nodes and Node Settings

If you add the Precursor Detector node to a workflow, place the node before the search engine node (that is, the Sequest HT node or the MSPepSearch node). These workflows include the Precursor Detector node:

PWF_Hybrid_Precursor_Quan_and_LFQ_MPS_SequestHT_Percolator.pdProcessingWF

PWF_Hybrid_MPS_SequestHT_INFERYS_Rescoring_Percolator.pdProcessingWF

PWF_Hybrid_MPS_SequestHT_INFERYS_Rescoring_Percolator_2stage.pdProcessingWF

For more information, see the Precursor Detector node in the Proteome Discoverer Help.