Before you run the Daemon utility from the Xcalibur data system, follow these procedures to set up the interface between the Daemon utility and the Xcalibur data system.

Procedure

  1. Before you start the Daemon utility, install the Proteome Discoverer application on a remote computer to decouple data processing from data acquisition.
  2. Thermo Fisher Scientific strongly recommends that you perform data analysis and data recording on two different computers to avoid disturbing the data acquisition by resource-consuming data processing.
  3. Install the Daemon utility on the same computer where the Xcalibur data system is running.
  4. Start the Proteome Discoverer application.
  5. In the Proteome Discoverer application, create the workflow to be used by the Daemon utility. See Create a workflow.
  6. After you install the Daemon utility, the Proteome Discoverer application places the directory where it saves the raw data files and stores the results in the following folder:
  7. Drive:\ProgramData\Thermo\Proteome Discoverer <release_number>\PublicFiles
  8. This directory might be invisible to you because the drive:\Documents and ProgramData directory is hidden. To display hidden directories, open File Explorer and select View. In the Show/hide area, select the Hidden items checkbox.
  9. (Optional) To change this directory for easier data access, open the Proteome Discoverer application, select Administration > Configuration, select Discoverer Daemon beneath Server Settings in the Configuration area in the left pane, and change the directory in the New Directory box.
  10. The settings are applied after you restart the Proteome Discoverer application.
  11. Change in the destination directory where results from the Daemon utility are stored