Cleavage enzyme specificities - Proteome Discoverer Software - Cleavage enzyme specificities - Proteome Discoverer Software - Proteome Discoverer Software

The application supports the following cleavage specificities for enzymes:

• Full: Specifies a full enzymatic digestion.
• Semi: Specifies a semi-enzymatic digestion. The cleavage is specific at one terminal end.
• Semi (N-Term): Specifies that the cleavage be specific at the amino terminal end and unspecific at the carboxyl terminal end.
• Semi (C-Term): Specifies that the cleavage be specific at the carboxyl terminal end and unspecific at the amino terminal end.
• Enzymatic Unspecific: Specifies an unspecific digestion with an extra weight for enzymatically digested peptides.

The list of cleavage reagents includes the No-Enzyme and No-Cleavage reagents, which are defined as follows:

• The No-Enzyme reagent digests a sequence at every amino acid. For example, if the FASTA file contains the sequence ABCDEFGHIK, the No-Enzyme reagent cleaves it into the following peptides: A, AC, ACD, ..., C, CD, CDE, ..., D, DE, DEF, and so on.
• The application supports the following specificity for the No-Enzyme reagent:
• - Unspecific: Specifies a non-specific digestion.

• The No-Cleavage reagent does not cleave a sequence. That is, the full sequence is the only possible sequence.
• The application supports the following cleavage specificities for the No-Cleavage reagent:
• - No-Cleavage: Specifies that no cleavages occur, so intact proteins result.

• Semi, Semi-N, Semi-C: Specifies terminal signal peptides that are cleaved in vivo.