The XlinkX algorithm identifies chemically crosslinked peptides. The Proteome Discoverer application supports different types of crosslinkers, mainly MS‑cleavable crosslinkers that are fragmented by the CID/HCD fragmentation method and non‑cleavable crosslinkers that do not break into signature fragments in the mass spectrum.

NOTE

For a full description of the XlinkX algorithm and the associated MS-based methods, refer to Liu, Fan, et al. 2017. Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification. Nat. Commun. (8) 15473. You can access this paper from the following link: https://www.nature.com/articles/ncomms15473.

The XlinkX algorithm is included in new processing and consensus workflow nodes. For information on these nodes, refer to the XlinkX Quick Start Guide.

The XlinkX algorithm requires a separate license. The Third-Party Installer software includes a 60-day demonstration version.