Reporter ion quantification - Proteome Discoverer Software - Reporter ion quantification - Proteome Discoverer Software - Proteome Discoverer Software

Reporter ion quantification, also known as isobarically labeled reporter ion quantification, uses reagents, or tags, to chemically label proteins and peptides. Reporter ion quantification uses tags that have the same mass, but produces fragments with different mass in the MS/MS spectrum that can be used for quantification. (A reporter ion is a fragment ion produced from the MS/MS of a peptide with an attached tag.)

Proteome Discoverer supports reporter ion quantification Tandem Mass Tag™ (TMT) quantification and isobaric tag for relative and absolute quantification (iTRAQ).

When you run tandem mass spectrometry (MS/MS) scans, the application performs identification and quantification with both TMT and iTRAQ.

The following is a list of quantification methods:

• TMT quantification offers default 2plex, 6plex, 10plex, 11plex, and TMTpro™ (16plex and 18plex) quantification methods.
• iTRAQ offers 4plex and 8plex quantification methods.

You can also add new methods by deriving them from an existing method.

For more information about TMT reagents, go to the following websites:

• https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-mass-spectrometry-analysis/protein-quantitation-mass-spectrometry/tandem-mass-tag-systems.html
• https://www.analyteguru.com