You already selected a consensus workflow in the New Study and Analysis dialog box. In this section, you configure some settings in the workflow nodes.

Procedure

  1. In the Analysis pane, select Edit on the Consensus Step.
  2. In the Workflow Tree pane, select the Precursor Ions Quantifier node.
  3. Change the Reject Quan Results with Missing Values parameter to True.
  4. NOTE

    This sample was set up to always have a 1:2:4 ratio for lysine and a 4:2:1 ratio for arginine; it is expected that all peptides will have quantitative values for each channel. For real biological samples, keep the default value, False.

  5. In the Protein Annotation Node, select Escherichia coli (strain K12) (sp_canonical TaxID=83333_and_subtaxonomies) (v2022-04-30).fasta in the Protein Database parameter.