1. In the Study, select the Grouping & Quantification tab.
  2. Under the Study Variables pane, select the E coli (ng) option.
  3. In the Bulk Ratio Generation pane, select E coli (ng): 96 and E coli (ng): 192.
  4. Select Add Ratios.
  5. There are now two ratios (192/96) in the Generated Ratios pane.
  6. In the Analysis pane, select Run.
  7. The processing and consensus workflows appear in the Job Queue.