Procedure
- Select the Workflows tab.
- Select Edit next to the Processing Step within the Analysis section.
- Select the CHIMERYS node in the Workflow Tree.
- For the Protein Database parameter, select the human FASTA file and the E. coli FASTA file.
- These are the files you added in Add the FASTA file to Proteome Discoverer.
NOTE
If using the CHIMERYS workflow, you will need to choose the Human and E coli databases, ensure that inferys_3_0_fragmentation is selected for the Prediction Model, Oxidation (M) is selected as a dynamic modification, and Carbamidomethyl (C) is selected as a static modification.