Quan method | TMT 10plex |
|---|---|
Quan channels | 126, 127N, 127C = Control 128N, 128C, 129N = Insulin stimulation 129C, 130N, 130C = IGF-1 stimulation 131 = Pool of control, insulin, and IGF-1 |
Organism | Human |
Instrument | Orbitrap Fusion Lumos |
Acquisition method | TMT SPS MS3 (CID MS2, HCD MS3) |
RAW file | 20160307_A549_Phospho_1ug_CID_NL_trigger_top1_Lumos_01.raw Located in the application media in the folder: Example RAW Files. Copy to a local directory. |
Sample description | A single TMT 10plex experiment with three replicates each of a human cell line under control, insulin stimulated, and IGF-1 stimulated conditions as well as one pooled sample; and uniform enrichment for phosphopeptides |
Disk space | 1GB local drive space, SSD drive preferred |
Processing time | Approximately 30 minutes |
For more information about the experiment, refer to the related application note, A2025, “Sensitive and accurate quantitation of phosphopeptides using TMT isobaric labeling techniques” at: https://www.analyteguru.com/.