In this section, you will configure some settings in the workflow nodes.

Prerequisites

  1. You already selected a consensus workflow in the New Study and Analysis dialog box. See Create the study.

Procedure

  1. In the Analysis section, select Edit or anywhere in the Consensus Step.
  2. In the Workflow Tree section, select the Reporter Ions Quantifier node.
  3. Change the Apply Quan Value Corrections parameter to True.
  4. In the Protein Annotation node, select the same protein database used in the Sequest HT search.
  5. Select the Grouping & Quantification tab.
  6. In the Study Variables section, select the Yeast Strain checkbox.
  7. In the Bulk Ratio Generation pane, select the Yeast Strain: Parental checkbox, and then select Add Ratios.
  8. In the Generated Ratios pane, confirm that the following entries appear:
  9. Met6/Parental
  10. His4/Parental
  11. Ura2/Parental
  12. In the Analysis pane, select Run.
  13. The processing and the consensus workflows appear in the Job Queue.