In this section, you will configure some settings in the workflow nodes.
Prerequisites
- You already selected a consensus workflow in the New Study and Analysis dialog box. See Create the study.
Procedure
- In the Analysis section, select Edit or anywhere in the Consensus Step.
- In the Workflow Tree section, select the Reporter Ions Quantifier node.
- Change the Apply Quan Value Corrections parameter to True.
- In the Protein Annotation node, select the same protein database used in the Sequest HT search.
- Select the Grouping & Quantification tab.
- In the Study Variables section, select the Yeast Strain checkbox.
- In the Bulk Ratio Generation pane, select the Yeast Strain: Parental checkbox, and then select Add Ratios.
- In the Generated Ratios pane, confirm that the following entries appear:
- Met6/Parental
- His4/Parental
- Ura2/Parental
- In the Analysis pane, select Run.
- The processing and the consensus workflows appear in the Job Queue.