Set up the processing workflow - Proteome Discoverer Software - Set up the processing workflow - Proteome Discoverer Software - Proteome Discoverer Software

Prerequisites

1. You already selected a processing workflow in the New Study and Analysis dialog box. See Create the study.

Procedure

1. Select the Workflows tab.
2. In the Analysis section, select Edit in the header of the Processing Step.
3. In the Workflow Tree, select the Spectrum Files RC node.
4. Select the Parameters of Spectrum Files RC tab.
5. For the Protein Database parameter, select the yeast FASTA file.
6. This is the file you added in Add the FASTA file to Proteome Discoverer.
6. Select the Sequest HT node in the Workflow Tree.
7. For the Protein Database parameter, select the yeast FASTA file, Saccharomyces cerevisiae.