ProSightPD supports analysis of heavy labeled proteoform data analysis. Specifically, ProSightPD supports the following heavy labeling:
- C13 labeling of carbon atom
- N15 labeling of Nitrogen
- Combined N15 labeling of Nitrogen and C13 labeling of carbon
The ProSightPD application assumes 100% labeling efficiency. Any incomplete labeling results in reduced performance.
Procedure
- To analyze heavy labeled data, do the following:
- In the Processing Workflow, select the cRAWler node in the Workflow Tree. The following image shows the ProSightPD High/High cRAWler node.
- In the Heavy Labeled parameter, select the correct label from the dropdown list.
NOTE
This applies to all data analyzed, therefore, unlabeled data is not compatible with a workflow using the heavy label parameter.