ProSightPD supports analysis of heavy labeled proteoform data analysis. Specifically, ProSightPD supports the following heavy labeling:

  • C13 labeling of carbon atom
  • N15 labeling of Nitrogen
  • Combined N15 labeling of Nitrogen and C13 labeling of carbon

The ProSightPD application assumes 100% labeling efficiency. Any incomplete labeling results in reduced performance.

Procedure

  1. To analyze heavy labeled data, do the following:
  2. In the Processing Workflow, select the cRAWler node in the Workflow Tree. The following image shows the ProSightPD High/High cRAWler node.
  3. In the Heavy Labeled parameter, select the correct label from the dropdown list.
  4. NOTE

    This applies to all data analyzed, therefore, unlabeled data is not compatible with a workflow using the heavy label parameter.