Procedure
- Create a new study or use an existing study.
- Select New Analysis to build a workflow consisting of only a Processing Step as shown in the following figure.
- Select the Workflows tab.
- The Workflow Tree opens.
- Drag the ProSightPD FASTA Exporter node to the Workflow Tree as shown in the following figure.
- Select Add Files. Add any RAW data file.
NOTE
The data file will not be processed, however, a raw file must be present to run the workflow.
- Select the Workflows tab and select the ProSightPD FASTA Exporter node.
- In the Protein Database parameter, select the database to be exported.
- In the Output File Name, enter the name for the exported database.
- Select Run.
NOTE
The FASTA database exports to the ProSightPD Database folder and must be imported through the ProSightPD Database Manager to create a correctly formatted Top-down database.
This process is only applicable for exporting FASTA databases from Proteome Discoverer. To import any FASTA formatted file, please see this section.
All FASTA file should be in Uniprot format (https://www.uniprot.org/help/fasta-headers).The ProSightPD FASTA Exporter node generates a FASTA file in Uniprot format.