To display the sequence maps for selected proteoforms in the results view, select Show ProSightPD Fragment Map

. These sequence maps are annotated with modifications and fragments from experimental MS/MS data.

If the ProSight Lite application is not already installed, install it from the prosightlite.northwestern.edu website.

By selecting PSLite in the External Top-Down Displays column or from the ProSightPD Fragment Map, you can view the fragment map associated with the match in the ProSight Lite application, as shown in the following figure.

Figure Associated fragment map

ProSightPD has a new feature that shows PSM ambiguity in the ProSightPD Fragment Map. The fragment map shows dashed boxes around the potential residues for modification with the potential sites of modification listed underneath. The following figure shows an example of a proteoform with two localized acetylation modifications in the middle of the sequence with a third acetylation that could either be on the N-terminus or on the third lysine.

Figure ProSightPD Fragment Map example with PSM ambiguity