The Quantifier node applies normalization and statistics to the resulting quantitative information. To use the volcano plots and heat maps, this node must be present in the consensus workflow.

The following table lists the node parameters.

Table ProSightPD Quantifier node parameters

Parameter

Description

1. Settings

Normalize

If set to True, normalization is applied to the proteoform level quantitation values.

Use Shared Results

If set to True, proteoforms can share quantification values.

Due to minor differences in modification states (for example, positional isomers) frequently similar proteoforms coelute and therefore share elution profiles. If non-similar, non-coeluting species utilize shared quantitation values, Thermo Fisher Scientific recommends lowering the feature mapping tolerances or setting Used Shared Results to False.