The Top-Down Search workflow

The Top-Down Search workflow is designed for deconvolution and identification of proteoform masses using the top-down search engine.

The Top-Down Search workflow contains the TD Search #1 and Results tabs.

Create a Top-Down Search workflow

From the Home screen, select the Top-Down Search workflow.
Load a data file and select spectra scans.
Select the Select from Available Databases button, and select or add an appropriate database for the analysis.
Select Deconvolution Settings and adjust the parameters if needed.
Process the data as follows:
1. Select a region on the Chromatogram to start the processing.
  The resulting masses display as a list under Proteoforms.
2. To change the display or specify selections for deconvolution, select Options in the Spectrum viewer.
3. Adjust the Search Settings if needed.
4. To zoom, select anywhere on the chromatogram and drag the mouse.
5. To add a custom mass, select Add Mass.
6. To search results in a database, select Search Results.
7. To change the view of spectrum, select m/z, Mass, or Mass and m/z.
In the Results tab, select Add to add a new set of results for the Top-Down search.

Note: Select Remove to delete a set of data you do not need for the Top-Down search. However, the last subunit cannot be removed by selecting this button.