Deconvolute and review data

Procedure

Under the Browse button in the Intact Masses pane, select Deconvolute.
ProSight Native performs mass deconvolution using the new settings and displays the results in the Spectrum panel.
Left-click and drag across the spectrum to zoom in to all the proteoform mass peaks in one charge state, for example +26.
Select the Options tab in the Spectrum panel to display additional features, such as swapping between light mode and dark mode and smoothing.
Select the magnifying glass icon on the top-right of the Spectrum panel to open a side-by-side view of the m/z and deconvoluted spectrum.
The Spectrum Viewer dialog box displays.

Note: This view also displays in the Mass and m/z tab in the Spectrum panel.
View the data grid under the Intact Masses panel to see tabular results for all of the deconvoluted proteoforms detected, including mass, intensity, and delta mass.

Note: Place your mouse cursor on the panel separator and drag it to the left to resize and view the entire data grid.

Note: The Sequence ID, Mods ID, delta mass for Intact Mass Tags (ΔM IMT), and IMTs columns are empty. The ΔM calculation is updated based on which row is selected in the table. See Perform an Intact Mass Search for information on filling them in.