Once the processing of an HDX experiment is complete, the application generates a set series of .csv files containing the HDX experiment results and stores them within the original raw data file folder in a new folder named "ExperimentNameHDXResults". You can open these .csv files in an Excel spreadsheet and plot the data as needed.
The table below describes the data contained in the HDX experiment output files.
File name (.csv) | Description |
|---|---|
HDX100ControlData | Deuterium contents in the 100% deuteration control sample. |
HDX100ControlModel | Deuterium contents in the 100% deuteration control sample compared to the values predicted by the back-exchange model. |
HDXDataxofy* | Raw deuterium content data for all conformation conditions. |
HDXSimulatedxofy* | Raw deuterium content data compared to the values predicted by the best HDX model. |
Solutionxofy* | Best solutions (protection factor values) for each conformation condition. |
ProtectionFactorPlot | Average of the top 20 possible solutions (protection factor values) for all conditions. |
ProtectionFactorDifferentialxofy* (For multiple conformation conditions only) | Top 20 differential log(protection factors) values, as well as their average values, of each condition compared to the control condition "A". |
DifferentialProtectionPlot (For multiple conformation conditions only) | Differences in the average possible log(protection factors) values compared to the control condition "A". |
OptimizedAverageSolution | The average value of the best 20 solutions (protection factor values) optimized by the application. Note: This solution is often better than any of the top 20 solutions. |
TimeCourses | Time-course data corrected for back-exchange. |
HDXData1stApproximationIonxofy* | Time-course data with corrected using internal standards, but without back-exchange correction. |
*For file names denoted by "xofy", when the experiment contains only one conformation condition, "xofy" reads "1of1". When the experiment contains two conformation conditions,"xofy" will read "1of2" and "2of2", and so on. | |