The following table describes the parameters in the Theoretical Digestion Results table on the Process and Review page for Peptide Mapping Analysis experiments.
Column | Description |
|---|---|
Alias Name | Displays the name of a theoretical digestion component using the alias root defined in the Theoretical Digestion and Alias Naming table. For example, if “Chain1” was typed into the Custom Chain Alias Root column of the Theoretical Digestion and Alias Naming table, the theoretical digestion components listed in the Theoretical Digestion and Alias Naming Results table will appear as “Chain1_x”, where x indicates the theoretical component number. |
Selected Protein Sequence Chain Name | Indicates the default name (derived from the FASTA file) of the protein sequence assigned to the currently open processing method. |
Sequence | Displays the amino acid sequence constituting the theoretical digestion component. |
Digested Sequence Mass | Displays the mass of the amino acid sequence constituting the theoretical digestion component. |
Start Position/End Position | Indicates the positions of the first and last residues of the theoretical digestion component within the undigested target protein sequence. |
Miscleavage | Indicates the number of miscleavages (if any) involved in producing a theoretical digestion component. The values in this column are based on the Number of Allowed Missed Cleavage Sites parameter in the Theoretical Digestion and Alias Naming section of the Customize Results page. If the value in this column is 0, no miscleavages occurred to create the theoretical digestion component. |
Digest Position | Indicates the relative position of a particular amino acid residue at which the theoretical digestion took place; based on the specific activity of the protease assigned to the theoretical digestion and intrinsically related to the theoretical component number listed in the alias name. For example, since trypsin cleaves the peptide bond between the C-terminal of a lysine (K) or an arginine (R) residue and an adjacent amino acid, for a theoretical tryptic digestion, a digest position of 8 indicates that the theoretical digestion component resulted from trypsin acting at the eighth K/R residue in the target protein sequence. For alias root “Chain1”, this theoretical component would have the alias name “Chain1_8”. |