On the Coverage page, the Coverage chromatogram pane displays a color-coded base peak chromatogram (BPC) depicting the protein coverage results for the experiment.

Prerequisites

  1. You have loaded the experiment results are viewing the Coverage page under the Mapping tab.

Procedure

  1. In the Coverage Results table, click the row of a protein, raw data file, or component.

  2. TIP

    Use the CTRL key to select the rows for multiple proteins in the experiment simultaneously.

  3. If you click a row at the protein (top) level, the Coverage chromatogram displays the protein coverage results for from the first (or only) raw data file in the experiment.
  4. (For experiments with multiple raw data files) If you click a row at the raw data file (second) level, the Coverage chromatogram displays the protein coverage results from the specified raw data file.
  5. If you click a row at the component (lowest) level, the Coverage chromatogram still displays the protein coverage results for the associated raw data file (see above) but displays the selected ion chromatogram (SIC) for the selected component below the BPC.
  6. The Coverage chromatogram updates, displaying the color-coded identified and unidentified proteins in the experiment. To change the color-coded shading options in the chromatogram, see Modify the shading parameters.
  7. image/svg+xml
    Figure Chromatogram pane zoomed in showing color-coded protein coverage
  8. In the Coverage chromatogram, the x axis represents the retention time range and the y axis indicates the relative intensity. Peaks are labeled with their individual retention times (by default).
  9. Each protein row selected in the Coverage Results table corresponds to one shading color on the chromatogram. The shading is semi-transparent so that you can view co-eluting peaks on top of each other.
  10. The height of the shaded block indicates the relative intensity (peak height) of a component. You can view this height value in the MS Height column at the component level in the Coverage Results table.
  11. If some component peaks are not identified, the application groups them together as the "Unidentified" protein type.
  12. image/svg+xml
    Figure Coverage chromatogram showing identified (red, green) and unidentified (blue) proteins
  13. For information regarding the commands available in the Coverage Chromatogram pane, see Coverage Chromatogram pane commands.