The Protein Sequence pane displays all of the protein chains in your analysis. When you select an identified component in the Results table, the corresponding amino acid sequence for the component is highlighted in yellow in the Protein Sequence pane.
Procedure
- Open the results of your experiment from the Queue page or from the Load Results page.
- See Open results.
- The Process and Review page opens by default and displays the results of your Peptide Mapping Analysis experiment, including the protein sequence.
- On the left side of the Process and Review page, select the Protein Sequence tab.
- Do one of the following:
- Select the row of an identified component in the Results table.
- For an experiment with multiple raw data files, expand (+/− icon) a component row in the Results table and select the row for a single raw data file.
- The peptide sequence corresponding to the component selected in the Results table appears highlighted in yellow in the Protein Sequence pane.
- If a component containing a disulfide bond is selected in the Results table, both peptide sequences involved in the disulfide bond appear highlighted in yellow in the Protein Sequence pane.
Figure Protein Sequence pane, displaying two chains
The Protein Sequence pane displays the following:
- All of the chains in the protein sequence, including the comment lines from the FASTA file that begin with the greater-than sign (>) to distinguish each chain.
- The amino acids in the sequence, divided into groups of ten and separated by spaces.
- The position labels, in groups of ten, displayed above the last amino acid in each group.
NOTE
Host cell proteins (HCPs) identified in the experiment do not appear in the Protein Sequence pane.