For a hydrogen deuterium exchange (HDX) experiment within the Peptide Mapping Analysis workflow, the HDX plots for the experiment are displayed on the Hydrogen Deuterium Exchange page. The following procedure describes how to navigate to the Hydrogen Deuterium Exchange page to view the HDX results.

For more detailed information about the plots on the Hydrogen Deuterium Exchange page, see Mapping > Hydrogen Deuterium Exchange page.

Prerequisites

  1. For an HDX-enabled Peptide Mapping Analysis experiment, you have processed your experiment and are viewing the results on the Process and Review page.

Procedure

  1. From the Process and Review page, select the Mapping tab in the navigation bar.
  2. The Mapping page opens, displaying the Coverage subpage by default.
  3. Select the Hydrogen Deuterium Exchange subtab.
  4. This subtab appears only when you enable the HDX feature on the Hydrogen Deuterium Exchange page of the processing method editing wizard.
  5. The Hydrogen Deuterium Exchange subpage opens, displaying an HDX plot for each chain in the protein sequence used for the HDX experiment, with the title of each chain displayed in red above the plot.
  6. image/svg+xml Chain Name Purple color and symbol for condition A Green color and symbol for condition B
    Figure HDX plots on the Hydrogen Deuterium Exchange page
  7. For an experiment with only one raw data file, the plot shows only one HDX trace (one color and symbol).
  8. For an experiment with multiple raw data files, the plot shows a different color and symbol for each condition, with the color/symbol legend at the upper right of the plot.
  9. The numbers at the top of each plot represent the residue numbers in the sequence.
  10. The letters at the bottom of each plot represent the amino acids in the sequence.
  11. The left side of the plot displays the protection factor range for the entire plot. Each data point on each HDX trace shows the protection factor value for a particular amino acid and residue.