In the Peptide Mapping Analysis workflow, the BioPharma Finder application performs component detection, peptide identification, and quantification of post-translational modifications (PTMs) for proteolytically digested proteins and peptides. Use this workflow to analyze your biotherapeutic using data-dependent LC/MS/MS or full-scan LC/MS data acquired on Thermo Scientific instruments.
The BioPharma Finder Peptide Mapping Analysis workflow comprises several key features, described in the table below.
Feature | Description |
|---|---|
Analyze sequence variants | Detect and characterize potential sequence variants in your biotherapeutic by performing a specialized, error-tolerant mass search against identified components. |
Identify unexpected components using de novo sequencing | Use de novo sequencing to identify unexpected contaminants or product-related impurities that can pose concerns regarding bioactivity, stability, and immunogenicity of your biotherapeutic. |
Map disulfide links | Characterize disulfide links in your biotherapeutic using the optimized Disulfide Bond Default Method processing method, which requires no prior knowledge of potential disulfide link sites. |
Identify N- and O-linked glycans | Detect and characterize glycosylation sites in your biotherapeutic from an extensive library of N- and O-linked glycans. |
Identify host cell protein (HCP) impurities | Perform an HCP database search to identify and characterize residual protein impurities in your biotherapeutic sample. |
Create and process an HDX experiment within the Peptide Mapping Analysis workflow to gain insight into the structure and conformational dynamics of your biotherapeutic. | |
Reprocess an experiment using real-time optimization (RTO) | Modify parameters and reprocess your experiment directly from the Process and Review page, where you can view updated results in real-time. |
Export results as a target peptide workbook | Save all or selected component results as a specially-formatted target peptide workbook for use with the Chromeleon chromatography data system or targeted Peptide Mapping Analysis experiments in the BioPharma Finder application. |
Prepare and customize reports | When connected to the Thermo Scientific Ardia Platform, create and share fully customizable data reports for analysis and quality control. |