The Method Editor application enforces these data-dependent scan rules to prevent illogical instrument methods:

  • The first data-dependent scan must be a data-dependent selected ion monitoring (ddSIM) scan or a data-dependent MS/MS (ddMS2) (level n = 2) scan.
  • No ddSIM or ddMS2 scan can follow a tMSn (level > 2)
  • Only a ddSIM or a ddMS2 scan can follow a ddSIM scan.
  • Only a ddMSn or a ddMS(n+1) scan can follow a ddMSn scan.
  • You can have Top N data-dependence for a ddMSn scan by using the Number of Scans setting. You can specify the amount of time or the number of scans to take in a data-dependent node.