The Method Editor application enforces these data-dependent scan rules to prevent illogical instrument methods:
- The first data-dependent scan must be a data-dependent selected ion monitoring (ddSIM) scan or a data-dependent MS/MS (ddMS2) (level n = 2) scan.
- No ddSIM or ddMS2 scan can follow a tMSn (level n > 2)
- Only a ddSIM or a ddMS2 scan can follow a ddSIM scan.
- Only a ddMSn or a ddMS(n+1) scan can follow a ddMSn scan.
- You can have Top N data-dependence for a ddMSn scan by using the Number of Scans setting. You can specify the amount of time or the number of scans to take in a data-dependent node.