The table below lists all known issues that exist in the Orbitrap Astral Series 2.0 SP1 Instrument Control Software release.

Table Known Issues

ID

Severity

Title

347288

4 - Low

Tune: Setting incorrect Syringe Pump flow rate displays wrong message “the syringe may be out of sample”.

Remedy: Remove and place the syringe again into the pump to release the backpressure.

357067

3 - Medium

Isolation m/z range below m/z 40 does not trigger a validation error when resulting from an inappropriate isolation offset setting.

Remedy: Enter an isolation offset value that complies with accepted isolation range.

387627

4 - Low

RF values are not converted to account for differences between S-lens and funnel when importing OE120/240/MX methods.

Remedy: Please manually adjust the RF value accordingly.

404844

3 - Medium

Xcalibur: If the file name contains the symbol μ, two raw data files are generated instead of one.

Remedy: Do not use symbol μ in file name.

614321

3 - Medium

Ion Foil Correction and Astral Mass Stabilization can fail due to uncalibrated ICS source.

Remedy: Perform System Calibration.

676639

3 - Medium

FAIMS: Temperature differences above 20 °C between inner and outer electrode might not be reachable and cause timeouts.

Remedy: When using "User Defined" FAIMS Mode in Method Editor, adjust FAIMS Inner Electrode Temp/FAIMS Outer Electrode Temp in order keep the temperature difference between the two electrodes below 20 °C.

813657

3 - Medium

Acquisition time not properly reported in rawfile with tMS2-only method not starting acquisition at t=0 min.

Remedy: Accept the incorrect report of acquisition time in rawfile (not affecting acquisition) or modify the method so that actual acquisition is started at t=0 min (e.g., by including a full scan experiment starting at t=0 min).

820630

3 - Medium

UI: ME: tSIM and tMS2: setting maxIT and microscans in table/scan property.

Remedy: Revert "Maximum Injection Time" or "Microscan" as a scan property.

824228

3 - Medium

DDA performed in small molecule application mode and with m/z of selected precursor ion varying substantially may result in unstable TIC for full scan MS and ddMS2 overfilling (for high mass precursor ion).

Remedy: Restrict the full scan mass range by using, ideally, a last m/z value not exceeding five times the first m/z value.

827732

2 - High

UI: ME: TMT HR Mode available in Small Molecule and Intact Protein Modes.

Remedy: Do not use TMT HR Mode in "Small Molecule" and "Intact Protein" modes as it is not relevant.

814458

3 - Medium

Scan Range mode set to "Auto-Extended" in some of the scans of a formerly saved method (using small molecule application mode) is changed to "Define m/z Range" when re-opened.

Remedy: The method works as expected at the time of saving. If the method is re-opened for modification, make sure Scan Range mode is set again to "Auto-Extended" before saving the method.

829920

3 - Medium

Methods including eDR m/z windows having first mass higher than m/z 2500 with "eDR Window Type" set to "Auto" do not report error and can be saved without warning, but do not pass method validation when executing a sequence in Xcalibur.

Remedy: Set "eDR Window Type" to "User Defined" before saving the method to confirm m/z window values are in accepted range.

830065

3 - Medium

Raw data files acquired using DIA methods with ''DIA Window Mode'' set to "Center Mass" report DIA m/z windows table with headers "m/z" and "Q1 Resolution (m/z)" instead of "Center Mass (m/z)" and "Window Width (m/z)", respectively, in summary section.

845079

3 - Medium

Service Diagnostic Tool running in the background can cause interruption in sequence execution

Remedy: Close Service Diagnostic Tool after service and troubleshooting tasks.