In the Oligonucleotide Analysis workflow, the BioPharma Finder application performs component detection, nucleotide identification, and quantification of post-translational modifications (PTMs) for oligonucleotides. Use this workflow to analyze your nucleotide-based therapeutic using data-dependent LC/MS/MS or full-scan LC/MS data acquired on Thermo Scientific instruments.
The BioPharma Finder Oligonucleotide Analysis workflow comprises several key features, described in the table below.
Feature | Description |
|---|---|
Quantify modifications | Detect and characterize modified components in the oligonucleotide sequence and compare ratios of modified to unmodified oligonucleotide in you sample. |
Reprocess an experiment using real-time optimization (RTO) | Modify parameters and reprocess your experiment directly from the Process and Review page, where you can view updated results in real-time. |
Use the Oligo Workbook and Calculator | Along with all or selected experiment results, generate and customize theoretical oligonucleotide components and save them to a specially-formatted oligo workbook that can be shared with the Chromeleon chromatography data system. |
Prepare and customize reports | When connected to the Thermo Scientific Ardia Platform, create and share fully customizable data reports for analysis and quality control. |