The MAM 2.0 workflow requires that you have installed and configured either the Thermo Scientific Vanquish Horizon UHPLC system or the Vanquish Flex Binary UHPLC system. The system performance evaluation test (SET) also requires that the system is equipped with a Thermo Scientific™ Hypersil GOLD™ VANQUISH™ C18 UHPLC column.
Follow the operating manual to complete the installation and configuration of your UHPLC system and to prepare all accessories and consumables. For details on setting up the instrument method in Chromeleon to run an analysis, see Setting up the instrument method.
NOTE
For optimal results upon method transfer, use the same configuration of your UHPLC system during the protein characterization phase and during the PQA monitoring phase.