The following table describes the information in the Results table in the Oligonucleotide Calculator and Workbook Editor.
Column | Description |
|---|---|
Sequence level | |
+/− | Select to show or hide the lower level of charge state information related to the current component row. |
Row number (bolded) | The number assigned to each visible workbook row in the table. This sequential numbering does not change when you sort or filter the table. |
(Checkbox) | Select this checkbox to export a workbook entry. To select or clear all of the checkboxes at once, select or clear the checkbox in the column header. If you filter the table, the following occurs:
|
Level | Indicates that the row is displaying experimental or theoretical sequence information (top level). |
Name | (Editable) Displays the name of the oligonucleotide sequence (derived from the FASTA file). |
Sequence | Displays the oligonucleotide sequence (using single-letter nucleotide notation) constituting the component. |
Sequence Triplet | Displays the oligonucleotide sequence (using triplet notation) constituting the component. |
Monoisotopic Mass [M] | Displays the experimental monoisotopic mass for a component. This mass is based on the exact mass of the most abundant isotope of each element in a molecule. |
Average Mass [M] | Displays the experimental average mass for a component. This mass is based on the average atomic weight of each element in a molecule. |
Formula | Displays the chemical formula of the neutral oligonucleotide component. |
Sequence Length | Indicates the number of nucleotides in the oligonucleotide component. |
Modification | Displays the type(s) of modification(s) associated with an identified component. For a description of the notation used in the "Mod" column, see Modification notation. If there is no modification, this column displays “None”. If a component is identified but does not adhere to the expected activity of the ribonuclease you selected, this column displays “Nonspecific”. The modifications listed in this column may include the variable modifications assigned during processing, nonspecific modifications, and other modifications determined automatically by the application. In this case, the application lists variable modification first, followed by static modifications in parentheses. Multiple modifications are separated by commas (“,”). |
Site | Displays the nucleotide base location of the modification(s) listed in the Modification column. The application lists the sites for variable modifications first, followed by the sites for static modifications in parentheses. Multiple modifications are separated by commas (“,”). If the application cannot identify the exact site of a modification or if there are multiple candidate residues within a component, the application indicates an approximate modification site with a tilde (“~”). |
RT Start | Displays the start time point of the retention time (RT) range for the component peak. |
RT Stop | Displays the end time point of the retention time (RT) range for the component peak. |
Charge state level | |
+/− | Select to show or hide the lower level of isotope information related to the current component row. |
Row number (bolded) | The number assigned to each visible charge state row in the table. This sequential numbering does not change when you sort or filter the table. |
Level | Indicates that the row is displaying charge state information (lower level). |
Charge state | Displays the charge state of a component. Charge state refers to the imbalance between the number of protons and the number of electrons in a molecular species (or an ion adduct). |
m/z | Displays the mass-to-charge ratio of the calculated monoisotopic mass for the given charge state. |
Monoisotopic Mass [M] M+Na* | Displays the monoisotopic mass of the oligonucleotide component as a monovalent sodium (Na+) adduct. *This column appears only when the "Adducts" checkbox is selected in the Optional Columns section. |
Monoisotopic Mass [M] M+2Na* | Displays the monoisotopic mass of the oligonucleotide component as a divalent sodium (2 Na+) adduct. *This column appears only when the "Adducts" checkbox is selected in the Optional Columns section. |
Monoisotopic Mass [M] M+K* | Displays the monoisotopic mass of the oligonucleotide component as a monovalent potassium (K+) adduct. *This column appears only when the "Adducts" checkbox is selected in the Optional Columns section. |
Monoisotopic Mass [M] M-A** | Displays the monoisotopic mass of the oligonucleotide component after the loss of a single adenine (A) base. If there are no A bases in the original sequence, this loss is not possible and the column displays "0.0000". **This column appears only when the "M−B" checkbox is selected in the Optional Columns section. |
Monoisotopic Mass [M] M-C** | Displays the monoisotopic mass of the oligonucleotide component after the loss of a single cytosine (C) base. If there are no C bases in the original sequence, this loss is not possible and the column displays "0.0000". **This column appears only when the "M−B" checkbox is selected in the Optional Columns section. |
Monoisotopic Mass [M] M-G** | Displays the monoisotopic mass of the oligonucleotide component after the loss of a single guanine (G) base. If there are no G bases in the original sequence, this loss is not possible and the column displays "0.0000". **This column appears only when the "M−B" checkbox is selected in the Optional Columns section. |
Monoisotopic Mass [M] M-T** | Displays the monoisotopic mass of the oligonucleotide component after the loss of a single tyrosine (T) base. If there are no T bases in the original sequence, this loss is not possible and the column displays "0.0000". **This column appears only when the "M−B" checkbox is selected in the Optional Columns section. |
Monoisotopic Mass [M] M-U** | Displays the monoisotopic mass of the oligonucleotide component after the loss of a single uracil (U) base. If there are no T bases in the original sequence, this loss is not possible and the column displays "0.0000". **This column appears only when the "M−B" checkbox is selected in the Optional Columns section. |
Isotope level | |
Row number (bolded) | The number assigned to each visible isotope row in the table. This sequential numbering does not change when you sort or filter the table. |
Level | Indicates that the row is displaying isotope information (lowest level). |
Num | Displays the number of isotopes for the given component. This number is set in the Save Oligo Workbook As dialog box when saving a workbook and can be changed in the Parameters section of the Oligo Calculator and Workbook Editor. |
m/z | Lists the mass-to-charge ratios for the isotopes. |