Import a FASTA file containing an oligonucleotide sequence - BioPharma Finder Software - Intact Mass Analysis User Guide - Create oligonucleotide sequences - Import a FASTA file containing an oligonucleotide sequence - BioPharma Finder Software - BioPharma Finder Software

Procedure

1. On the BioPharma Finder Home page, in the Experiment Types pane, select the Sequence Manager link.
2. The Sequence Manager opens.
2. On the right side of the Sequence Manager, select the New Oligonucleotide button.
3. The Oligonucleotide Sequence Editor opens.

4. 

3. In the Oligonucleotide Sequence Editor, select Import Fasta File on the command bar.
4. The Open dialog box opens.
4. Locate and select the FASTA file (.fasta) you want to import and select Open.

5. NOTE

   FASTA files must not exceed 1 MB for import.

6. If the entered chain contains invalid oligo building blocks or an invalid format, an error message appears.
7. The Oligo Sequence Map on the left side of the page updates and displays the added chain.
8. The name of the chain appears above the chain sequence, indicated by a greater-than sign (>) to distinguish each chain.
9. Additionally, the read-only information fields (Monoisotopic Mass, Average Mass, and Formula) in the Sequence Information pane > Target Oligonucleotide area update to reflect the properties of the entire protein.
10. In the Sequence Information pane > Chain area, you can view the Monoisotopic Mass and Average Mass properties for an individual chain by selecting the chain number from the dropdown menu.
5. On the left side of the Oligonucleotide Sequence Editor, in the Sequence Information pane > Target Oligonucleotide area, enter the following information:
1. Name: Type the name of the oligonucleotide sequence.
2. Description: (Optional) Type a description for the oligonucleotide sequence.
3. Category: Select the category for the oligonucleotide sequence from the dropdown menu.
6. Save the oligonucleotide sequence.
7. The application adds the saved sequence to the Sequence Manager table.