Use the Random Sequence Generator in the Oligonucleotide Sequence Editor to generate and save randomized sequence variations of your oligonucleotide sequence. These randomized sequences are analyzed along with the original oligonucleotide sequence so that you can confidently identify and analyze the component results displayed on the Process and Review page.

Prerequisites

  1. You are editing an oligonucleotide sequence in the Oligonucleotide Sequence Editor.

Procedure

  1. In the Oligonucleotide Sequence Editor, in the Sequence Information pane > Random Sequence Generator area, type the number of randomized sequence variations you want to generate in the Random Sequence box.

  2. NOTE

    You can create up to five randomized sequences using the Random Sequence Generator.

  3. Select Apply.
  4. The Oligo Sequence Map pane displays both the original oligonucleotide sequence and the randomized sequences.

  5. NOTE

    Each randomized sequence is named using the original oligonucleotide name appended with “Randomized” and the corresponding randomize sequence number.

    For example, for the original oligonucleotide sequence “15mer”, the first randomized sequence would be named “15mer_Randomized_1”.

  6. Random Sequence Generator in the Oligonucleotide Sequence Editor
    Figure Random Sequence Generator in the Oligonucleotide Sequence Editor
  7. Save the oligonucleotide sequence.
  8. The application adds the saved sequence to the Sequence Manager table.