If the Enable Drug-to-Antibody Ratio checkbox is selected in the processing method for an Intact Mass Analysis experiment, the Average DAR pane on the Process and Review page is visible. The Average DAR pane displays the calculated average drug-to-antibody ratio (DAR) values using all of the components that have a drug load identification. To update these calculated values, you can enter a different drug load value or select a different identification in the Matched Sequence pane, or select a different set of components in the Results table.

Procedure

  1. Open the results of your experiment from the Queue page (for Automatic mode experiments) or from the Load Results page (for either Automatic of Manual mode experiments).
  2. See Open results.
  3. The Process and Review page opens by default and displays the results of your Intact Mass Analysis experiment, including the average DAR values (if applicable).
  4. On the Process and Review page, select the Average DAR tab (tabbed together with the Source Spectra and Matched Sequence panes) on the right side of the page.
  5. The Average DAR pane opens and displays the Average DAR and Component Specific Summary tables.
  6. image/svg+xml
    Figure Average DAR pane displaying the DAR values and components used in the calculations
  7. The Average DAR table displays the experiment- and raw data file-level average DAR results. See Average DAR table parameters.
  8. The Component Specific Summary displays the component-level DAR results. See Component Specific Summary table parameters.
  9. The table lists only the components used in the DAR calculations. You can export the data from this table. See Average DAR pane commands.
  10. (Optional) To change the calculated average DAR values for the experiment, do any of the following:
  11. In the Matched Results pane, select the row of a drug load component in the Matched Results table. Next to the Drug Load value, select the Override checkbox, modify the value, and then press the ENTER key.
  12. The application recalculates the DAR values based on your modified drug load and updates the tables in the Average DAR pane.
  13. The Results table and the drug load label on the spectra in the Deconvoluted Spectrum pane also automatically update with the modified drug load value.
  14. In the Review table, select the row for a drug load component and change the matched sequence identification on the Matched Results pane.
  15. See Change the matched sequence identification for a component.
  16. The application recalculates the DAR values based on your modified drug load and updates the tables in the Average DAR pane.
  17. The Results table and the drug load label on the spectra in the Deconvoluted Spectrum pane also automatically update with the modified drug load value.
  18. Select or clear the checkboxes for the component rows in the Results table and then select Recalculate in the top right corner of the Average DAR pane.
  19. If you select one or more component rows, the application recalculates the average DAR values using only the selected components and updates the values in the Average Drug-to-Antibody Ratio table.
  20. If you clear all of the components in the Results table, the application automatically recalculates the average DAR values in the Average Drug-to-Antibody Ratio table using only the default components that have a drug load identification.
  21. The Component Specific Summary table at the bottom of the Average DAR pane also updates to show only the list of components used in the calculations.
  22. (Optional) After you make modifications to the identifications and DAR calculations, select Save Results As in the command bar of the Process and Review page to save to a new experiment or overwrite the current experiment results.